The collected data of the PhD Thesis of Richards, Nicholas John 2025

How to cite this Dataset

Richards, Nicholas John (2025): The collected data of the PhD Thesis of Richards, Nicholas John 2025. University of Reading. Dataset. https://doi.org/10.17864/1947.001405

Description

This is a dataset of the data generated over the course of the PhD studies of Nicholas John Richards (2020-2024). It includes methods, analysis, figures, and example representative images. Much of the data deposited here are the microscope images in the form of TIFF files alongside the Excel sheets and GraphPad Prizm files that record, statistically analyse, and graph the data involved in the production of the figures of the above-mentioned thesis and published paper which resulted. The figures also included contained in PowerPoints. It is arranged by thesis chapter and experiment or stain performed. The complete methods used are also included in Word documents in a folder named as such.

The thesis by Nicholas John Richards to which this data relates is entitled: “The effects of the venom of the Tarantula spiders Poecilotheria regalis and Thrixopelma pruriens on skeletal muscle damage and nociception.” One paper has been published, this paper is Richards et al (2023), Indian Ornamental Tarantula (Poecilotheria regalis) Venom Affects Myoblast Function and Causes Skeletal Muscle Damage.

It was contributed to by Pradeep Vijaya Kumar (University of Reading, https://orcid.org/0000-0002-9894-2201) who collected the biochemistry data relating to the P. regalis venom (fluorescent PLA2, serine protease and metalloprotease assays) but these were analysed and graphed by Nicholas John Richards. The caseinolytic assays and biochemical assays on T. pruriens venom were performed by Nicholas John Richards.
Adedoyin Balogun (Hull York Medical School) also contributed by taking the images of the F4/80 staining of macrophages, these appeared as supplementary figures. These assay data and images were also later included in my thesis, with credit given, so are also included here. The copyright for the F4/80 images remains with Adedoyin Balogun. In addition, a further, at this time (March 2025) untitled, short communication is being drafted on the topic of nociception caused by the P. regalis venom.

Some data about an unrelated snake venom is the work of Sodiq Adeyemi (SA) (University of Reading, https://orcid.org/0000-0002-7664-7340). It is included because we used the same plate to investigate the caseinolytic activity of the P. regalis and the Bitis arietans Puff Adder snake. To avoid edits to the files and changes to the date modified this is also included.

Resource Type:

Dataset

Creators:

Richards, Nicholas John