Ultrahigh-throughput LAP-MALDI MS analysis of peptides and an enzyme assay

Description

As part of the PhD project 'Advancing liquid MALDI Ion Source Designs and Applications in Modern Biological Mass Spectrometry' funded by the University of Reading and Waters Corporation the possiblity for high-throughput analysis of biomolecules was investigated.

Raw and processed data for liquid atmospheric-pressure matrix-assisted laser desorption/ionisation (LAP-MALDI) mass spectrometry analysis of peptides and enzyme assay at speeds greater than 20 samples/s using a hardware and software-modified Synapt G2-Si with a UV laser are presented.

The deposited data relate to the research article 'Ultrahigh-throughput sample analysis using liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry', 2022, Krenkel et al., https://doi.org/10.1021/acs.analchem.1c05614.

Resource Type:	Dataset
Creators:	Krenkel, Henriette ORCID: https://orcid.org/0000-0001-6553-401X and Cramer, Rainer ORCID: https://orcid.org/0000-0002-8037-2511
Contributors:	Brown, Jeffery ORCID: https://orcid.org/0000-0001-8569-7174, Richardson, Keith, Hoyes, Emmy and Morris, Michael
Rights-holders:	University of Reading
Data Publisher:	University of Reading
Publication Year:	2021
Data last accessed:	3 December 2024
DOI:	https://doi.org/10.17864/1947.000338
Metadata Record URL:	https://researchdata.reading.ac.uk/id/eprint/338
Organisational units:	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Participating Organisations:	University of Reading, Waters Corporation
Keywords:	mass spectrometry, LAP-MALDI, high-throughput, liquid atmospheric-pressure matrix-assisted laser desorption/ionisation
Rights:	Creative Commons Attribution 4.0 International
Data Availability:	OPEN
Additional details Additional details Project Name: Advancing liquid MALDI ion source designs and applications in modern biological mass spectrometry Funders: Waters Corporation Resource Language: English Data collection method: The dataset contains data that was generated using a commercial mass spectrometer Synapt G2-Si HDMS (Waters) equipped with an in-house developed AP-MALDI ion source. Masslynx (ver. 4.2; Waters) software was used to collect and process the data. ToF calibration was performed post acquisition on [Glu1]-fibrinopeptide B fragments. Data processing and preparation activities: Further processing making use of 2 applications developed by the authors (which can be obtained on request) and SpecProc (see https://sourceforge.net/projects/specproc/) and SpecProc SampleList (see https://sourceforge.net/projects/specproc-samplelist/). Depositing User: Henriette Krenkel Date Deposited: 22 Nov 2021 10:06 Last Modified: 29 Jul 2023 04:18