1. ABOUT THE DATASET ------------ Title: Residue dynamics of cyprodinil and fludioxonil in pollen and nectar of courgette (Cucurbita pepo L.) Creator(s): Fiona Gierer Organisation(s): University of Reading Rights-holder(s): University of Reading Publication Year: 2022 Description: The aim of this study was to investigate and compare the residue profile in pollen, nectar, flowers, anthers, and leaves of courgette plants following a foliar spray application of two pesticides with contact and systemic properties. Therefore, glasshouse experiments were set up. Courgette plants were sprayed during flowering with cyprodinil and fludioxonil. Samples were taken daily over the course of two weeks and were analysed for residues of both active ingredients using LC-MS/MS. Cite as: Gierer (2022): Residue dynamics of cyprodinil and fludioxonil in pollen and nectar of courgette (Cucurbita pepo L.). University of Reading. Dataset. https://doi.org/10.17864/1947.000375. Contact: fiona.gierer@outlook.com 2. TERMS OF USE ----------------- Copyright 2022 University of Reading. This dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/. 3. PROJECT AND FUNDING INFORMATION ------------ Title: Improving our understanding of residues in bee important matrices Dates: September 2017 - November 2021 Funding organisation: University of Reading and Syngenta Ltd (Jeallot's Hill, Bracknell, UK) Grant no.: Not available 4. CONTENTS ------------ File listing ResidueData-Courgette-Experiment1: Residue data of fludioxonil and cyprodinil in courgette pollen, nectar, anthers, flowers and leaves following a foliar spray application during flowering collected on 0-13 days after application (DAA) ResidueData-Courgette-Experiment2: Residue data of cyprodinil in courgette pollen and nectar following translocation within the plant collected on 0 - 10 days after application (DAA) 7 variables are included in each dataset: Sample (sample name), Repetition (treatment replicate), DAA (days after application sample was taken), DoseRate (in kg active ingredient/ha), Residues (in mg/kg), Matrix (plant matrix analysed), AI (active ingredient applied/analysed) 5. METHODS -------------------------- Courgettes (Cucurpita pepo var. cylindrica) were grown in pots in the glasshouse. Experiment 1: During flowering, the pots were gathered in a randomised block design with four replicates, with each block consisiting of three treatment plots: One treatment plot consisted of 10 plants each. The first treatment was an application of fludioxonil at an application rate of 500 g ai/ha. The second treatment was an application of cyprodinil at an application rate of 750 g ai/ha. The third treatment was left without any pesticide application and served as control plot. Active ingredients (ai) were applied using a hand-held sprayer during flowering. Samples of pollen, nectar, anthers, flowers and leaves were taken 0 days after application (0 DAA) from each plot, when the spray solution had dried, and on each subsequent day for 2 weeks. All flowers in one plot were cut and nectar was removed using a pipette and stored in Eppendorf tubes. Pollen was carefully scraped off with a plastic stick into Eppendorf tubes. About 10g of leaves were randomly collected in each plot. All samples were stored at -18 degree Celsius until analysis. For experiment 2, instead of an overhead foliar spray application, cyprodinil was applied to the leaves only using a small spray bottle when the plants were in full flower. Each plant received 0.96mL of spray solution with a concentration of 68 mg ai mL−1, which corresponds to the theoretical amount of ai a plant has received in experiment 1. Pollen and floral nectar were collected for 10 days as described above. Samples were extracted twice with acetonitrile/water (80/20, v/v) on a tissue homogeniser (FastPrep, MP Biomedicals), ultrasonicated and centrifuged. Analysis was performed using LC-MS/MS. Matrix-matched standards were used for calibration. At least one control, one reagent blank and two procedural recoveries at the LOQ (0.01mg/kg) and 10xLOQ were included in each run which were within 70-120% with and RSD<20%. Matrix effects and linearity (R2>0.99) were assessed. Control samples were free from contamination, but are not included in the dataset. -