Dataset supporting 'The use of salts, buffers and surfactants in LAP-MALDI MS'

How to cite this Dataset

Krenkel, Henriette (2023): Dataset supporting 'The use of salts, buffers and surfactants in LAP-MALDI MS'. University of Reading. Dataset. https://doi.org/10.17864/1947.000464

Description

As part of the PhD project 'Advancing liquid MALDI ion source designs and applications in modern biological mass spectrometry' funded by the University of Reading and Waters Corporation the influence of salts, buffers, surfactants and other substances on the LAP-MALDI MS signal of a peptide mix was investigated.

The peptide mix containing leucine enkephalin, angiotensin I, bradykinin, substance P and melittin was mixed with substances at different concentrations. Those were: 1) buffers (HEPES, MES, MOPS, tris, tricine, PBS), 2) salts (chlorides of calcium, potassium, magnesium, sodium, ammonium and acetates of magnesium, ammonium and sodium), 3) surfactants (sodium dodecyl sulphate, sodium deoxycholate, octyl-beta-D-glucopyranoside, CHAPS, amidosulfobetamine-14), 4) ammonium salts and serine (ammonium acetate, tartrate, citrate, phosphate, oxalate) and 5) other substances (EDTA, ammonium sulphate, urea, bovine serum albumin, formic acid, trifluoracetic acid and dimethyl sulfoxide).

All samples were analysed at least in triplicate with a water-based sample as reference. For the LAP-MALDI analysis a CHCA-based liquid support matrix was used and samples were analysed on a Synapt G2-SI.

The data archived here include the raw data and sample locations.

Resource Type:

Dataset

Creators:

Krenkel, Henriette