1. ABOUT THE DATASET ------------ Title: Dataset supporting 'Effects of food matrix on the prebiotic efficacy of inulin-type fructans: a randomised trial' Creator(s): Peter Jackson (https://orcid.org/0000-0003-0098-4719) Organisation(s): University of Reading Rights-holder(s): Beneo GmbH Publication Year: 2023 Description: This dataset was collected as part of my PhD thesis titled 'An exploration of the prebiotic concept: from food matrix to mood state'. This data was collected from healthy individuals in the Berkshire area aged 18-65. The aim of this study was to determine the effect of the food matrix on the prebiotic efficacy of inulin-type fructans. The study design was a 10-day, 4-arm, parallel, randomised trial involving 96 adults. Volunteers were evaluated for eligibility into the trial and if deemed acceptable were assigned into one of four groups. Each group was assigned a different intervention: either pure inulin or inulin-enriched milk chocolate, rice drink or shortbread. Volunteers provided baseline stool and urine samples and were asked to consume their products twice daily for the entire 10-days of the intervention. Volunteers then provided both stool and urine samples at completion. Changes in faecal microbial load and composition were analysed via fluorescence in situ hybridisation and 16S rRNA sequencing. Urine samples were analysed via 1H-NMR and SIMCA software. In addition, throughout the course of the intervention volunteers were asked to complete daily gastrointestinal sensation and bowel habit diaries, as well as dietary questionnaires. Gastrointestinal sensation and bowel habit data were collected using REDCap. Nutrient data was captured using the validated eNutri database. The eNutri database is validated food frequency questionnaire database designed to capture and analyse changes in diets over both short and long-term periods. The eNutri database was developed at the University of Reading. Further details on the eNutri database can be found here - https://doi.org/10.1371/journal.pone.0214931 The dataset is in xlsx format. Cite as: Jackson, P. P. J., (2023): Data supporting 'Effects of food matrix on prebiotic efficacy of inulin-type fructans: a randomised trial'. University of Reading. Dataset. https://doi.org/10.17864/1947.000500 Related publication: Jackson et al., (2023). Effects of food matrix on the prebiotic efficacy of inulin-type fructans: a randomised trial. Beneficial Microbes, 14(4). pp. 317-334, DOI: https://doi.org/10.1163/18762891-20220120 Additional information can be found in my thesis: Jackson, Peter (2023). An exploration of the prebiotic concept: from food matrix to mood state. Volume I: Effect of food matrix on the prebiotic efficacy of inulin-type fructans - Chapter 3: Effect of food matrix on the prebiotic efficacy of inulin-type fructans – human intervention study. PhD thesis, University of Reading, DOI: https://doi.org/10.48683/1926.00117328 Contact: Prof Bob Rastall, Department of Nutrition and Food Science, University of Reading mail: r.a.rastall@reading.ac.uk 2. TERMS OF USE ------------ Copyright Beneo GmbH 2023. This dataset is available to authorised users subject to a Data Access Agreement between the University of Reading and a recipient organisation. A copy of the University of Reading Data Access Agreement is included with this item. To request access to the database, please complete a data access request at https://redcap.link/data-request. 3. PROJECT AND FUNDING INFORMATION ------------ Title: Effect of food matrix on prebiotic efficacy of inulin-type fructans Dates: 2019-2023 Funding organisation: Beneo-Institute, Beneo GmbH, Obrigheim, Germany 4. CONTENTS ------------ File listing File name: Food_Matrix_Dataset.xlsx The meta dataset contains the following individual level participant information: - Participant metrics age, height, weight, BMI - Nutrient data - Total kcals, Total CHO, Total Protein, Total fat, PUFAs, Starch, Total sugar, Fiber (all expressed as grams per day) - GI sensations daily - Stool Frequency, Stool Consistency, Flatulence, Intestinal Bloating, Abdominal Pressure, Abdominal Pain, Feeling of Fullness - GI sensations average (D0-D5 and D6-D10) - Stool Frequency, Stool Consistency, Flatulence, Intestinal Bloating, Abdominal Pressure, Abdominal Pain, Feeling of Fullness - EUB I,II,II (Total bacteria counts) at D0 and D10 - Counts are expressed as Log10 cells/g wet faeces - Bif164 (Bifidobacterium spp) at D0 and D10 - Counts are expressed as Log10 cells/g wet faeces - Bac303 (Most Bacteroidaceae and Prevotellaceae, some Porphyromonadaceae) at D0 and D10 - Counts are expressed as Log10 cells/g wet faeces - Erec482 (Most of the Clostridium coccoides-Eubacterium rectale group - Clostridium cluster XIVa and XIVb) at D0 and D10 - Counts are expressed as Log10 cells/g wet faeces - Rrec584 (Roseburia spp) at D0 and D10 - Counts are expressed as Log10 cells/g wet faeces - Prop853 (Clostridium cluster IX) at D0 and D10 - Counts are expressed as Log10 cells/g wet faeces - Fprau655 (Faecalibacterium and relatives) at D0 and D10 - Counts are expressed as Log10 cells/g wet faeces - Sequencing 16S RMP (Relative Microbiome Profiling) data at genus level - counts are expressed as relative percentage - Sequencing 16S QMP (Quantitative Microbiome Profiling) data at genus level - counts are expressed as cell/gram faeces - 1-HMNR spectra data Key Abbreviations - IN = inulin - SHORT = shortbread - MC = Milk Chocolate - RD = Rice Drink - CHO = Total carbohydrates - PUFAs = polyunsaturated fatty acids FoodMatrix_Orginal_reads - Food_Matrix_fastq.zip - Contains all 16S rRNA fastq files for individual participants at D0 and D10 of the intervention Key - IN = inulin - SHORT = shortbread - MC = Milk Chocolate - RD = Rice Drink - D0 = Day 0 - D10 = Day 10 - S = sample no - R1 = Read 1 - R2 = Read 2 5. METHODS ----------- A detailed description of methodology can be found in my thesis as well in the published article. Please refer to the publication below: Jackson et al., (2023). Effects of food matrix on the prebiotic efficacy of inulin-type fructans: a randomised trial. Beneficial Microbes. 1-18, DOI: https://doi.org/10.1163/18762891-20220120