Dataset supporting a project of developing a nanoHPLC-MALDI MS/MS workflow employing CID of multiply charged proteolytic peptides for bottom-up proteomics

How to cite this Dataset

Ryumin, Pavel and Cramer, Rainer (2016): Dataset supporting a project of developing a nanoHPLC-MALDI MS/MS workflow employing CID of multiply charged proteolytic peptides for bottom-up proteomics. University of Reading. Dataset. https://doi.org/10.17864/1947.74

Description

Dataset contains two sets of LC-MALDI MS/MS runs performed on an in-house developed AP-MALDI source attached to a commercial mass spectrometer, as well as a complementary LC-ESI MS/MS dataset. Other data include experiments on AP-MALDI MS signal duration and MS/MS experiments.

Resource Type:

Dataset

Creators:

Ryumin, Pavel

and Cramer, Rainer

Rights-holders:

University of Reading

Data Publisher:

University of Reading

Publication Year:

2016

Data last accessed:

31 October 2025

DOI:

https://doi.org/10.17864/1947.74

Metadata Record URL:

https://researchdata.reading.ac.uk/id/eprint/74

Organisational units:

Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry

Participating Organisations:

University of Reading

Keywords:

LC-MALDI, liquid MALDI, AP-MALDI, bottom-up proteomics, multiply charged ions, MALDI CID MS/MS

Rights:

Creative Commons Attribution 4.0 International

Data Availability:

OPEN

Additional details

Project Name:	Development of a Novel MALDI Mass Spectrometer and Technology for the Generation of Multiply Charged Ions at High Sensitivity
Funders:	Engineering and Physical Sciences Research Council (EP/L006227/1)
Resource Language:	English
Data collection method:	The dataset contains data that was generated using a commercial mass spectrometer Synapt-G2Si (Waters) equipped with an in-house developed AP-MALDI ion source. Masslynx software (ver. 4.1; Waters) was used to collect and process the data. All data files contain a _HEADER file with relevant instrument settings and employed methods recorded. Manuscript in preparation at time of data deposit entitled 'A nanoHPLC-MALDI MS/MS workflow employing CID of multiply charged proteolytic peptides for bottom-up proteomics' by P.Ryumin, J.Brown, M.Morris, R.Cramer contains the methods section where the exact procedures of data acquisition are described.
Data processing and preparation activities:	Only the data starting with [ESI, FA, TFA] was processed to compensate systematic mass accuracy errors. For this the 'Modify Calibration' method within MassLynx software was used.
Depositing User:	Dr Pavel Ryumin
Date Deposited:	13 Jul 2016 08:06
Last Modified:	29 Jul 2023 04:18