This dataset includes all summaries of raw data supporting the results presented in the paper "Fish oil supplements, but not oily fish, alter the number and function of extracellular vesicles in healthy human subjects: A randomized, double-blind, placebo-controlled, parallel trial".The objective of this paper is to investigate the effects of fish oil supplements providing 2.2 g/d of n-3 polyunsaturated fatty acids (PUFAs) and oily fish at a level achievable in the diet providing 1.44 g/d of n-3 PUFA, on the numbers, compositions and procoagulant activity of extracellular vesicles (EVs) in healthy human volunteers. Data about EVs parameters include: i. the numbers, and size of circulating total EVs measured by Nanoparticle Tracking Analysis (NTA); ii. the numbers of EV subpopulations (i.e. phosphatidylserine-positive EVs (PS+EVs), platelet-derived EVs (PDEVs), endothelial-derived EVs (EDEVs)) by flow cytometry (FCM); iii. the procoagulatory activity of circulating EVs including in vitro thrombogenic potential of EVs in activating tissue factor-dependent thrombin generation measured by thrombin generation assay and  in vitro clot-forming capacity of EVs measured by clot formation and lysis assays. Data about fatty acid compositions of red blood cells (RBCs) and EVs were measured by gas chromatography. Data about plasma lipid profile include total cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and triacylglycerol concentrations measured by Randox. Data about subjects baseline characteristics include i. height, weight and body mass index (BMI) measured by Tanita; ii. blood pressure measured by upper arm blood pressure monitor.