The aim of the study was to investigate the pesticide residue profiles of the two active ingredients fludioxonil and cyprodinil in different sunflower matrices including pollen and nectar. Therefore, sunflower plants were sprayed during flowering and at different developmental stages to assess the influence of time on residue expression. Extrafloral nectar, pollen, anther, florets and leaf samples were taken daily over the course of 1-2 weeks. Samples were analysed for residues of both active ingredients using LC-MS/MS.