This data file contains the full gene expression values (fpkm) of 121 Eruca vesicaria subsp. sativa samples, obtained by RNAseq (performed by Novogene UK Ltd., Cambridge, UK). Plants were grown in two field trials, one in Rome, Italy, and the other in Dorchester, UK. Six E. vesicaria cultivars were sampled across two consecutive harvests (first and second cut) two weeks apart, and again across two shelf life time points (D0 and D5). The file contains expression values for 49,487 genes, their relative positions on the E. vesicaria reference genome (https://www.ncbi.nlm.nih.gov/bioproject/PRJEB50993), their strand orientation, length, and annotation.