Dataset supporting paper 'Dietary n-3 polyunsaturated fatty acids alter the number, fatty acid profile and coagulatory activity of circulating and platelet-derived extracellular vesicles'

How to cite this Dataset

Zhou, Ruihan and Bozbas, Esra (2023): Dataset supporting paper 'Dietary n-3 polyunsaturated fatty acids alter the number, fatty acid profile and coagulatory activity of circulating and platelet-derived extracellular vesicles'. University of Reading. Dataset. https://doi.org/10.17864/1947.000513

Description

This dataset includes all reports and summaries of raw data supporting the results presented in the paper "Dietary n-3 polyunsaturated fatty acids alter the number, fatty acid profile and coagulatory activity of circulating and platelet-derived extracellular vesicles". The objective of this paper is to investigate whether daily supplementation of participants at moderate risk of (cardiovascular diseases) CVDs with 1.8 g/d of fish oil-derived n-3 polyunsaturated fatty acids (PUFAs) altered the generation, composition and function of circulating and platelet-derived extracellular vesicles (EVs). Data about EVs parameters include: i. the numbers, and size of circulating total EVs and in vitro generated platelet-derived EVs (PDEVs) measured by Nanoparticle Tracking Analysis (NTA); ii. the numbers of EV subpopulations (i.e. phosphatidylserine-positive EVs (PS+EVs), PDEVs, endothelial-derived EVs (EDEVs)) and PS expression on PDEVs generated in vitro from platelets measured by flow cytometry (FCM); iii. the procoagulatory activity of circulating EVs (in vitro thrombogenic potential in activating tissue factor-dependent thrombin generation) measured by thrombin generation assay. The coagulatory behaviour of PDEVs generated in vitro from platelets measured by thrombin generation, clot formation, fibrinolysis and in vivo thrombus formation assays. Data about conventional cardiovascular risk markers include i. body mass index (BMI) measured by Tanita; ii. blood pressure measured by upper arm blood pressure monitor; iii. plasma lipid profile (i.e. triacylglycerol (TAG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and glucose concentrations measured by iLab. Data about thrombogenic markers include: i. plasma platelet aggregation measured by 96-well high-throughput aggregometry; ii. plasma thrombin generation measured by thrombin generation assay; iii. plasma clot growth and fibrinolysis measured by thrombodynamics analyzer.

Resource Type:

Dataset

Creators:

Zhou, Ruihan and Bozbas, Esra

Rights-holders:

University of Reading, Esra Bozbas

Data Publisher:

University of Reading

Publication Year:

2023

Data last accessed:

25 April 2024