University of Reading Research Data Archive

Exploring the prebiotic potential of cellobiose using in vitro batch fermentation gut model

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Description

This dataset contains the raw experimental data. The study investigated the prebiotic potential of commercial cellobiose compared to oligofructose P95 (FOS) using a pH-controlled, batch in vitro human faecal fermentation model (n=3 donors). The data includes Short-Chain Fatty Acid (SCFA) concentrations, and bacterial population enumeration via Fluorescent In Situ Hybridization (FISH).
The data supports the findings reported in the manuscript: 'Cellobiose as a candidate prebiotic: Enhanced butyrate production in an in vitro human gut fermentation model' This study is also presented as Chapter 5 of the PhD thesis: 'Extraction of cellobiose from spent coffee grounds and prebiotic potential of commercial cellobiose'.

Outcomes include:
• Bacteriology changes (Bacterial population enumeration via Fluorescent In Situ Hybridization (FISH)
• Metabolite changes (Short-Chain Fatty Acids via Gas Chromatography with downstream Flame Ionization Detector (GC-FID).

Resource Type: Dataset
Creators: Huang, Manxi ORCID logoORCID: https://orcid.org/0009-0005-7448-2393
Contributors: Rastall, Robert ORCID logoORCID: https://orcid.org/0000-0003-1775-5226 and Jackson, Peter ORCID logoORCID: https://orcid.org/0000-0003-0098-4719
Rights-holders: Manxi Huang
Data Publisher: University of Reading
Publication Year: 2026
Data last accessed: 12 March 2026
DOI: https://doi.org/10.17864/1947.001505
Metadata Record URL: https://researchdata.reading.ac.uk/id/eprint/1505
Organisational units: Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group
Participating Organisations: University of Reading
Keywords: cellobiose, batch fermentation model, gut microbiome
Rights:
Data Availability: OPEN

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